胶质细胞源性神经生长因子
GDNF配体家族
受体
离解常数
神经营养因子
配体结合分析
细胞生物学
化学
细胞培养
分子生物学
生物
生物化学
遗传学
作者
Luis Quintino,Aurélie Baudet,Jonas Larsson,Cecilia Lundberg
标识
DOI:10.1016/j.jneumeth.2013.04.006
摘要
Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cells allowed readily detection of GDNF binding and displacement to endogenous receptors. The dissociation constant and half maximal inhibitory concentration obtained were comparable to other studies using standard binding assays. Overall, this FACS-based, simple to perform and adaptable to high throughput setup, provides a safer and reliable alternative to radioactive methods.
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