生物膜
白色念珠菌
突变体
微生物学
生物
营养不良
白色体
质粒
拉伤
基因
分子生物学
细菌
生物化学
遗传学
解剖
作者
Tzu‐Ling Tseng,Wei Lai,Tai-Lin Lee,Wan Hua Hsu,Yu Wen Sun,Wan Chen Li,Chun‐Wen Cheng,Jia‐Ching Shieh
出处
期刊:Canadian Journal of Microbiology
[Canadian Science Publishing]
日期:2015-04-01
卷期号:61 (4): 247-255
被引量:5
标识
DOI:10.1139/cjm-2014-0526
摘要
The CDC4 gene is nonessential in Candida albicans and plays a role in suppressing filamentous growth, in contrast to its homologues, which are involved in the G1–S transition of the cell cycle. While characterizing the function of C. albicans CDC4 (CaCDC4), we found that the loss of CaCDC4 resulted in a reduction in cell flocculation, indicating a possible role for CaCDC4 in biofilm formation. To elucidate the role of CaCDC4 in biofilm formation, Cacdc4 null mutant strains were constructed by using the mini-Ura-blaster method. To create a CaCDC4 rescued strain, the plasmid p6HF-ACT1p-CaCDC4 capable of constitutively expressing CaCDC4 was introduced into the Cacdc4 homozygous null mutant. To determine the biofilm formation ability, an in vitro XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium-5-carboxanilide) reduction assay was used. Compared with the parental auxotrophic strain BWP17, the Cacdc4 homozygous null mutant was able to enhance biofilm formation significantly. This enhancement of biofilm formation in the Cacdc4 homozygous null mutant could be reversed by constitutively expressing CaCDC4. We conclude that CaCDC4 has a role in suppressing biofilm formation in C. albicans.
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