One new negative stiffness suspension is introduced in this paper. The vehicle with negative stiffness suspension has good ride comfort and handling stability. The natural frequency of system could be reduced in vertical direction by applying negative stiffness suspension. The vehicle model with negative stiffness suspension or not is built in ADAMS. The comparison of simulation results show that the vehicle with negative stiffness suspension could reduce the natural frequency of system and vibration transmissibility, and also improve the vehicle ride comfort and vehicle handling stability.