过氧亚硝酸盐
化学
二氯荧光素
荧光团
荧光
活性氮物种
活性氧
过氧亚硝酸
光化学
生物化学
超氧化物
量子力学
物理
酶
作者
Dan Yang,Huali Wang,Ziqi Sun,Nga‐Wai Chung,Jiangang Shen
摘要
We have found a specific reaction between ketone 1 and peroxynitrite (ONOO-), rather than other reactive oxygen species and reactive nitrogen species generated in the biological system. On the basis of this reaction, we have successfully developed a new fluorescent probe HKGreen-1, which is highly selective for the detection of peroxynitrite in living cells. Before the oxidation with peroxynitrite, the dichlorofluorescein part is masked and the probe is nonfluorescent. However, upon reaction with peroxynitrite, the fluorophore is released, resulting in strong enhancement in fluorescence intensity.
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