量子点
超氧化物
氧化应激
生物物理学
生物传感器
细菌
化学
材料科学
纳米技术
生物化学
生物
酶
遗传学
作者
Wasana Senevirathna,Ruth Kiro,Rachel Rosen,Inna Popov,Shimshon Belkin,Mona Wells
标识
DOI:10.1080/17435390802546089
摘要
A panel of genetically-engineered Escherichia coli biosensor bacteria, responsive to chemical stressors inducing heat shock, DNA damage, oxidative stress (peroxide- and superoxide-type) and fatty acid metabolism interference, was used to characterize the chemical effect of CdSe quantum dot exposure. Results implicate a primary mode of superoxide-type stress toxicity that is dependent upon quantum dot dose, size, and chain length of capping agent, and are consistent with results from other reports employing mammalian cell lines. Imaging studies confirm association of quantum dots with lipophilic regions of the biosensor bacteria (i.e., inner/outer membranes, cell wall, periplasmic space), and demonstrate intracellular alteration of the quantum dot lattice. Corroboratory experiments with superoxide stress mutants and antioxidant amendments confirm oxidative stress while also implicating destabilization of quantum dots.
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