基因组
蛋白质细菌
细菌
抗生素耐药性
水处理
氯
生物
微生物学
丰度(生态学)
16S核糖体RNA
微生物种群生物学
氯霉素
抗生素
环境化学
化学
环境科学
环境工程
生态学
基因
遗传学
有机化学
作者
Peng Shi,Shuyu Jia,Xuxiang Zhang,Tong Zhang,Shengfeng Cheng,Aimin Li
出处
期刊:Water Research
[Elsevier]
日期:2013-01-01
卷期号:47 (1): 111-120
被引量:411
标识
DOI:10.1016/j.watres.2012.09.046
摘要
This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dominating in the drinking water and chlorine disinfection greatly affected microbial community structure. After chlorination, higher proportion of the surviving bacteria was resistant to chloramphenicol, trimethoprim and cephalothin. Quantitative real-time PCRs revealed that sulI had the highest abundance among the antibiotic resistance genes (ARGs) detected in the drinking water, followed by tetA and tetG. Chlorination caused enrichment of ampC, aphA2, bla(TEM-1), tetA, tetG, ermA and ermB, but sulI was considerably removed (p < 0.05). Metagenomic analysis confirmed that drinking water chlorination could concentrate various ARGs, as well as of plasmids, insertion sequences and integrons involved in horizontal transfer of the ARGs. Water pipeline transportation tended to reduce the abundance of most ARGs, but various ARB and ARGs were still present in the tap water, which deserves more public health concerns. The results highlighted prevalence of ARB and ARGs in chlorinated drinking water and this study might be technologically useful for detecting the ARGs in water environments.
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