A highly disordered pregraphitic carbon nanofiber with the product of its quasi-Fermi wave vector and mean free path close to 1 was fabricated using electrospinning technique. Strong localization made the conductivity vary with temperature as lnσ∝T−1∕2 from 300to5K, suggesting variable range hopping as the conductivity mechanism, and resulted in a large negative magnetoresistance from 300K down to 1.9K that can still be quantitatively described using weak localization and electron interaction models.