脐静脉
p38丝裂原活化蛋白激酶
ICAM-1
VCAM-1
肿瘤坏死因子α
信号转导
化学
MAPK/ERK通路
细胞生物学
细胞间粘附分子-1
细胞粘附分子
磷酸化
U937电池
分子生物学
生物
生物化学
免疫学
细胞凋亡
体外
作者
Hongtao Liu,Wenming Li,Pei Hsin Huang,Wenjuan Chen,Qishun Liu,Bai X,Chao Yu,Yifeng Du
标识
DOI:10.1016/j.carbpol.2010.01.054
摘要
This study aimed to investigate the inhibitive effects of chitosan oligosaccharides (COS) on tumor necrosis factor (TNF)-α-induced over-expression of vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs). We found that COS effectively inhibited TNF-α-induced expression of VCAM-1 and ICAM-1 at the level of transcription and translation. Signal transduction studies suggested that COS blocked TNF-α-induced activation of NF-κB, degradation of IκBα, and phosphorylation of p38 MAPK and ERK1/2. A further investigation showed that the NF-κB activation can be partly suppressed by p38 MAPK inhibitor (SB203580) and ERK1/2 inhibitor (PD98059), which also ameliorated the mRNA expression of VCAM-1 and ICAM-1 in TNF-α-induced HUVECs. Additionally, COS decreased U937 monocyte adhesion to HUVECs induced by TNF-α. Our findings suggest that COS inhibit VCAM-1 and ICAM-1 production in activated HUVECs at least partly through the blockade of p38 and ERK1/2 signaling pathways.
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