Validation of ECoScreen 2 for Collection of fractionated Exhaled Breath Condensate (EBC)

Introduction: Systematic analysis of markers from exhaled breath condensate (EBC) has become a more and more interesting tool for non-invasive diagnosis of lung and airway diseases. Standardization of EBC collection with respect to collection time and breathing pattern has been achieved by the development of ECoScreen and ECoVent. However, the issues on the pulmonary site in regard to the release of different markers remains still unclear. The aim of the study was to provide the technical tool to assess whether EBC markers are released from the conducting airways or the lung periphery.