化学
荧光
酶
分子生物学
抗原
抗体
有孔小珠
免疫分析
生物化学
色谱法
生物
材料科学
量子力学
物理
复合材料
免疫学
作者
David M. Rissin,Cheuk W. Kan,Todd Campbell,Stuart C. Howes,David R. Fournier,Linan Song,Tomasz Piech,Purvish P. Patel,Lei Chang,Andrew J. Rivnak,Evan P. Ferrell,Jeffrey Randall,Gail K. Provuncher,David R. Walt,David C. Duffy
摘要
The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target protein molecules per bead) with an enzymatic reporter capable of generating a fluorescent product. After isolating the beads in 50-fl reaction chambers designed to hold only a single bead, we used fluorescence imaging to detect single protein molecules. Our single-molecule enzyme-linked immunosorbent assay (digital ELISA) approach detected as few as approximately 10-20 enzyme-labeled complexes in 100 microl of sample (approximately 10(-19) M) and routinely allowed detection of clinically relevant proteins in serum at concentrations (<10(-15) M) much lower than conventional ELISA. Digital ELISA detected prostate-specific antigen (PSA) in sera from patients who had undergone radical prostatectomy at concentrations as low as 14 fg/ml (0.4 fM).
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