色谱法
化学
蛋白质沉淀
分析物
样品制备
检出限
三级四极质谱仪
质谱法
分析化学(期刊)
选择性反应监测
串联质谱法
作者
Line Småstuen Haug,Cathrine Thomsen,Georg Becher
标识
DOI:10.1016/j.chroma.2008.10.113
摘要
A sensitive and reliable method based on column switching liquid chromatography (LC) coupled to a triple quadrupole mass spectrometer (MS) has been developed for quantification of 19 perfluorinated compounds (PFCs) in serum. A volume of only 150microl serum is used and protein precipitation by methanol is the only sample preparation necessary prior to injection into the column switching system. Pseudo-MRM is used as a detection mode for determination of PFCs, resulting in reduced background noise and considerably increased sensitivity for the perfluorinated alkyl sulfonates and the perfluorinated alkyl sulfonamides. The estimated limits of detection for the method were as low as 0.0020-0.050ngPFCs/ml serum. The accuracy determined from spiking experiments, reported as recovery of added amount, was between 85 and 121% in the range 0.20-50ngPFC/ml serum, except for perfluorodecylsulfonate for which the accuracy was 146% at 0.20ngPFC/ml serum. The low sample volume needed, the limited manual handling and the broad range of analytes which are included, make this method advantageous for large-scale epidemiological studies. This column-switching technique can easily be set up on standard LC-MS/MS instruments and is thus available to a wide range of laboratories.
科研通智能强力驱动
Strongly Powered by AbleSci AI