模板
DNA
聚合物
材料科学
涂层
航程(航空)
纳米技术
HMG盒
领域(数学分析)
生物物理学
DNA结合蛋白
化学
生物化学
生物
基因
转录因子
数学分析
复合材料
数学
作者
Armando Hernández-García,Nicole A. Estrich,Marc W. T. Werten,Johan R. C. van der Maarel,Thomas H. LaBean,Frits A. de Wolf,Martien A. Cohen Stuart,Renko de Vries
出处
期刊:ACS Nano
[American Chemical Society]
日期:2016-12-12
卷期号:11 (1): 144-152
被引量:49
标识
DOI:10.1021/acsnano.6b05938
摘要
Emerging DNA-based nanotechnologies would benefit from the ability to modulate the properties (e.g., solubility, melting temperature, chemical stability) of diverse DNA templates (single molecules or origami nanostructures) through controlled, self-assembling coatings. We here introduce a DNA coating agent, called C8–BSso7d, which binds to and coats with high specificity and affinity, individual DNA molecules as well as folded origami nanostructures. C8–BSso7d coats and protects without condensing, collapsing or destroying the spatial structure of the underlying DNA template. C8–BSso7d combines the specific nonelectrostatic DNA binding affinity of an archeal-derived DNA binding domain (Sso7d, 7 kDa) with a long hydrophilic random coil polypeptide (C8, 73 kDa), which provides colloidal stability (solubility) through formation of polymer brushes around the DNA templates. C8–BSso7d is produced recombinantly in yeast and has a precise (but engineerable) amino acid sequence of precise length. Using electrophoresis, AFM, and fluorescence microscopy we demonstrate protein coat formation with stiffening of one-dimensional templates (linear dsDNA, supercoiled dsDNA and circular ssDNA), as well as coat formation without any structural distortion or disruption of two-dimensional DNA origami template. Combining the programmability of DNA with the nonperturbing precise coating capability of the engineered protein C8–BSso7d holds promise for future applications such as the creation of DNA–protein hybrid networks, or the efficient transfection of individual DNA nanostructures into cells.
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