分泌物
化学
群体感应
VI型分泌系统
毒力
细胞生物学
绿脓素
突变体
大肠杆菌
周质间隙
响应调节器
作者
Anjali Y. Bhagirath,Sai Prasad Pydi,Yanqi Li,Chen Lin,Weina Kong,Prashen Chelikani,Kangmin Duan
出处
期刊:ACS Infectious Diseases
[American Chemical Society]
日期:2017-02-10
卷期号:3 (2): 162-175
被引量:24
标识
DOI:10.1021/acsinfecdis.6b00153
摘要
One of the leading causes of morbidity and mortality in cystic fibrosis (CF) patients is pulmonary infection with Pseudomonas aeruginosa, and the pathophysiology of pulmonary infection in CF is affected by the lifestyle of this micro-organism. RetS-GacS/A-RsmA is a key regulatory pathway in P. aeruginosa that determines the bacterium’s lifestyle choice. Previously, we identified PA1611, a hybrid sensor kinase, as a new player in this pathway that interacts with RetS and influences biofilm formation and type III secretion system. In this study, we explored the structural and mechanistic basis of the interaction between PA1611 and RetS. We identified the amino acid residues critical for PA1611–RetS interactions by molecular modeling. These residues were then targeted for site-directed mutagenesis. Amino acid substitutions were carried out at seven key positions in PA1611 and at six corresponding key positions in RetS. The influence of such substitutions in PA1611 on the interaction was analyzed by bacterial...
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