转录组
开放式参考框架
拟南芥
十字花科
核糖体分析
生物
翻译(生物学)
打开阅读框
细胞生物学
遗传学
计算生物学
信使核糖核酸
基因
植物
基因表达
突变体
肽序列
作者
Jinyan Wang,Yang Li,Mingyue Li,Wenting Zhang,Yaping Lü,Kai Hu,Xitie Ling,Tianzi Chen,Dongshu Guo,Youxin Yang,Zhe Zheng,Qing Liu,Baolong Zhang
标识
DOI:10.1021/acs.jafc.3c08528
摘要
Salt stress significantly impedes plant growth and the crop yield. This study utilized de novo transcriptome assembly and ribosome profiling to explore mRNA translation's role in rice salt tolerance. We identified unrecognized translated open reading frames (ORFs), including 42 upstream transcripts and 86 unannotated transcripts. A noteworthy discovery was the role of a small ORF, Ospep5, in conferring salt tolerance. Overexpression of Ospep5 in plants increased salt tolerance, while its absence led to heightened sensitivity. This hypothesis was corroborated by the findings that exogenous application of the synthetic small peptide Ospep5 bolstered salt tolerance in both rice and Arabidopsis. We found that the mechanism underpinning the Ospep5-mediated salt tolerance involves the maintenance of intracellular Na+/K+ homeostasis, facilitated by upregulation of high-affinity potassium transporters (HKT) and Na+/H+ exchangers (SOS1). Furthermore, a comprehensive multiomics approach, particularly ribosome profiling, is instrumental in uncovering unannotated ORFs and elucidating their functions in plant stress responses.
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