吹覆盆子
浆果
化学
哌替尼丁
保健品
食品科学
多酚
代谢组学
氰化物
芍药苷
花青素
色谱法
植物
抗氧化剂
马维定
生物
飞燕草素
生物化学
作者
Chamali Kodikara,Thomas Netticadan,Nandika Bandara,Champa Wijekoon,Srinivas Sura
标识
DOI:10.1016/j.foodchem.2024.138778
摘要
Phenolic compounds are considered an important group of bioactive molecules that are present in abundant quantities in fruits such as berries and cherries; hence, the analysis and quantification of these compounds are of significant interest to the scientific community. The current study aimed to develop a novel analytical method using liquid chromatography and high-resolution mass spectrometry (UHPLC-HRMS) for the rapid, comprehensive and simultaneous analysis of 66 phenolic compounds optimized for the selected five types of fruits commercially available in Canada. Bioactive compounds that could potentially be metabolite markers for each berry were identified. Various phenolic compounds were identified and quantified in all five selected fruits. Notably, blackberries were rich in anthocyanins such as cyanidin-3-glucoside (368.4 ± 6 µg/g), while blueberries were rich in peonidin-3-glucoside (1083 ± 9 µg/g). In addition, raspberries and cherries contained significant amounts of cyanidin-3-rutinoside, at 3156 ± 36 µg/g and 301.3 ± 2 µg/g, respectively, while cranberries contained the highest concentrations of petunidin at 829.7 ± 3 µg/g. The newly developed and validated UHPLC-HRMS method proved helpful in comprehensively analyzing phenolic compounds in blueberry, raspberry, cranberry, blackberry and cherry. Identifying and quantifying bioactives can lead to applications in neutraceutical and pharmaceutical industries by using phenolic-rich berry extracts in functional foods, supplements, or pharmaceutical products.
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