穗
赤霉素
水稻
突变体
串扰
花序
细胞生物学
生物
蛋白激酶A
植物
磷酸化
生物化学
基因
物理
光学
作者
Enyang Mei,Ming He,Min Xu,Jufen Tang,Jiali Liu,Yingxiang Liu,Zhang Bao Hong,Xiufeng Li,Zhenyu Wang,Qingjie Guan,Xiaojie Tian,Qingyun Bu
摘要
Panicle exsertion is one of the crucial agronomic traits in rice (Oryza sativa). Shortening of panicle exsertion often leads to panicle enclosure and severely reduces seed production. Gibberellin (GA) plays important roles in regulating panicle exsertion. However, the underlying mechanism and the relative regulatory network remain elusive. Here, we characterized the oswrky78 mutant showing severe panicle enclosure, and found that the defect of oswrky78 is caused by decreased bioactive GA contents. Biochemical analysis demonstrates that OsWRKY78 can directly activate GA biosynthesis and indirectly suppress GA metabolism. Moreover, we found OsWRKY78 can interact with and be phosphorylated by mitogen-activated protein kinase (MAPK) kinase OsMAPK6, and this phosphorylation can enhance OsWRKY78 stability and is necessary for its biological function. Taken together, these results not only reveal the critical function of OsWRKY78, but also reveal its mechanism via mediating crosstalk between MAPK and the GA signaling pathway in regulating panicle exsertion.
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