In vivo corrosion on retrieved hip endoprostheses and in vitro effects of corrosion products on bone mineralization

体内 腐蚀 碱性磷酸酶 矿化(土壤科学) 成骨细胞 植入 化学 生物相容性 冶金 材料科学 生物医学工程 体外 外科 生物化学 医学 生物 有机化学 氮气 生物技术
作者
Maria Herbster,Eva Müller,Jannik Jahn,Adrian Buchholz,Kaspar Tootsi,Christoph H. Lohmann,Thorsten Halle,Jessica Bertrand
出处
期刊:Bone [Elsevier]
卷期号:175: 116852-116852
标识
DOI:10.1016/j.bone.2023.116852
摘要

In vivo corrosion of modular endoprostheses remains a great concern, as the release of heavy metal ions can impair the implant's service life and the wellbeing of the patient. The detailed corrosion mechanisms that occur in vivo are so far not completely understood. In this context, the effects of implant released cobalt (Co) and chromium (Cr) ions on osteoblast mineralization and gene expression have not been investigated extensively. This comprehensive study aimed at furthering the understanding of in vivo implant corrosion from the clinical signs via prosthesis retrievals and histology of the synovial membranes down to the molecular processes instigated by corrosion products and its effects on bone mineralization. A detailed in vivo failure analysis was performed investigating 22 retrieved hip endoprostheses from different manufacturers and taper material combinations. The aim was to find a correlation of taper damage and especially corrosion to susceptible biomedical alloys and its effect on periprosthetic tissue as well as the clinical implant performance with regard to revision diagnosis and presence of radiolucent lines (RLL). A second part investigated the effects of Co and Cr ions on the in vitro mineralization process of osteoblasts. Cell cultures were exposed to relevant concentrations of CoCl2 and CrCl3 (0 μM, 100 μM, 200 μM) with and without addition of phosphate. Mineralization behavior was analyzed with Alizarin Red assay and Von Kossa staining of calcium depots, alkaline phosphatase activity of osteoblasts and gene expression was analyzed with real time quantitative PCR. The retrieval study provides evidence of in vivo fretting and crevice corrosion on all metallic tapers combined with either ceramic or metal femoral heads. Within the modular taper junctions, selective dissolution of the α phase occurred in wrought TiAl6V4 alloys, and etching of the fine-grained wrought CoCr28Mo6 alloy implants was observed in formed crevices. In addition, significant amounts of wear particles and corrosion products were detected in retrieved synovial membranes. An increased risk for the occurrence of a RLL in the proximal zones was determined for patients with a corroded mixed metal taper. Whereas Co ions have hardly any effects on mineralization, Cr ions cause a significant concentration dependent decrease in mineralization rate of osteoblasts. However, this effect is alleviated by addition of a phosphate source. Our data reveal that Cr ions depleted dissolved phosphates by forming an insoluble complex (CrPO4), which inhibits the phosphate dependent mineralization process. No significant effect of the heavy metal ions on osteoblast activity by means of alkaline phosphate activity as well as on gene expression is determined. This study broadens the understanding of in vivo corrosion of metallic modular implants and its clinically relevant effects on mineralization. Based on these findings, in vivo corrosion of CoCr28Mo6 endoprostheses should be limited to avoid inhibitory effects of Cr3+ on bone mineralization which can contribute to premature implant failure.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
rayqiang完成签到,获得积分10
3秒前
朱晖完成签到 ,获得积分10
16秒前
背后的秋柳完成签到 ,获得积分10
19秒前
Kevin完成签到,获得积分10
23秒前
晶晶在努力完成签到 ,获得积分10
27秒前
黯黑の夜完成签到,获得积分10
36秒前
友好亚男完成签到 ,获得积分10
38秒前
愿景完成签到 ,获得积分10
49秒前
爆米花应助科研通管家采纳,获得10
50秒前
南风完成签到 ,获得积分10
57秒前
郭义敏完成签到,获得积分10
57秒前
sally完成签到,获得积分10
1分钟前
sydhwo完成签到 ,获得积分10
1分钟前
xixi完成签到 ,获得积分10
1分钟前
xiaoruixue完成签到,获得积分10
1分钟前
BaooooooMao完成签到,获得积分10
2分钟前
燕山堂完成签到 ,获得积分10
2分钟前
2分钟前
mgqqlwq发布了新的文献求助10
2分钟前
李健的小迷弟应助mgqqlwq采纳,获得10
2分钟前
上官若男应助科研通管家采纳,获得10
2分钟前
科研通AI2S应助jlwang采纳,获得10
2分钟前
Noah完成签到 ,获得积分10
3分钟前
舒心小猫咪完成签到 ,获得积分20
3分钟前
唐唐完成签到,获得积分10
3分钟前
Singularity完成签到,获得积分0
3分钟前
月影碎星河完成签到 ,获得积分10
3分钟前
ii完成签到 ,获得积分10
3分钟前
Summer_Xia完成签到,获得积分10
3分钟前
叮叮当当完成签到,获得积分10
4分钟前
碳土不凡完成签到 ,获得积分10
4分钟前
橙子味的邱憨憨完成签到 ,获得积分10
4分钟前
4分钟前
没用的三轮完成签到,获得积分10
4分钟前
简单的战斗机完成签到,获得积分10
4分钟前
专注的玉米完成签到 ,获得积分10
4分钟前
xiaowuge完成签到 ,获得积分10
5分钟前
光亮乘云完成签到 ,获得积分10
5分钟前
laohu完成签到,获得积分10
5分钟前
jue完成签到 ,获得积分10
5分钟前
高分求助中
Un calendrier babylonien des travaux, des signes et des mois: Séries iqqur îpuš 1036
Sustainable Land Management: Strategies to Cope with the Marginalisation of Agriculture 1000
Corrosion and Oxygen Control 600
Python Programming for Linguistics and Digital Humanities: Applications for Text-Focused Fields 500
Heterocyclic Stilbene and Bibenzyl Derivatives in Liverworts: Distribution, Structures, Total Synthesis and Biological Activity 500
重庆市新能源汽车产业大数据招商指南(两链两图两池两库两平台两清单两报告) 400
Division and square root. Digit-recurrence algorithms and implementations 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2546081
求助须知:如何正确求助?哪些是违规求助? 2175672
关于积分的说明 5600244
捐赠科研通 1896383
什么是DOI,文献DOI怎么找? 946268
版权声明 565379
科研通“疑难数据库(出版商)”最低求助积分说明 503557