甜菜碱
蔗渣
木质素
化学
纤维素酶
纤维素
水解
酶水解
半纤维素
乳酸
食品科学
有机化学
生物化学
生物技术
细菌
生物
遗传学
作者
Ying Chen,Cuiluan Ma,Wei Tang,Yubin He
标识
DOI:10.1016/j.biortech.2023.129485
摘要
Green solvents, especially deep eutectic solvents (DESs), are widely applied to pretreat biomass for enhancing its enzymatic hydrolysis. In this work, lactic acid was selected as the hydrogen-bond-donor to prepare Betaine-base DES (Betaine:LA), The DES was utilized to pretreat sugarcane bagasse (SCB) at 160 ℃ for 80 min (severity factor LogR0 = 3.67). The influences of Betaine:LA treatment on the chemical composition, crystal and microstructure structure of cellulose, and cellulase digestion were investigated. The results showed that the lignin (47.1%) and xylan (44.6%) were removed, the cellulase digestibility of Betaine:LA-treated SCB was 4.2 times that of the raw material. This improved efficiency was attributed to the enhanced accessibility of cellulose, the weakened surface area of lignin, the declined hydrophobicity, and the decreased crystallinity of cellulose. Several compelling linear correlations were fitted between enzymatic hydrolysis and these alterations of physicochemical features, comprehensively understanding enzymatic saccharification of Betaine:LA-pretreated SCB.
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