牙周膜干细胞
微泡
牙周纤维
细胞生物学
MAPK/ERK通路
生物
小RNA
碱性磷酸酶
外体
污渍
间充质干细胞
磷酸化
生物化学
医学
酶
牙科
基因
作者
Chen Lin,Yingying Yang,Yingxue Wang,Heng Jing,Xinyi Bai,Hong Zheng,Chunxiang Zhang,Hui Gao,Linkun Zhang
标识
DOI:10.1016/j.yexcr.2022.113318
摘要
Several studies have confirmed that exosomes containing microRNAs (miRNAs) from the aseptic inflammatory microenvironment play an important role in bone remodeling. But the mechanism that induces changes in the osteogenic ability of periodontal ligament stem cells (PDLSCs) is still unclear. In the present study, the osteogenic function of periodontal ligament fibroblasts-derived exosomes induced by PGE2 on PDLSCs was detected by real-time PCR, alizarin red assay and alkaline phosphatase staining. High-throughput miRNAs sequencing was used to reveal that miR-34c-5p in exosomes-PGE2 was upregulated compared it in exosomes-normal. Real-time PCR and western blotting assay verified that overexpression of miR-34c-5p inhibited osteogenic differentiation, and reduced phosphorylation of ERK1/2. In addition, dual-luciferase reporter assay revealed that miR-34c-5p targeted special AT-rich sequence-binding protein 2 (SATB2). It was shown that exosomal miR-34c-5p inhibited osteogenic differentiation of PDLSCs via SATB2/ERK pathway.
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