Phytoconstituents of Indian mustard honey impart antidepressant activity in reserpine-induced depressed condition through activation of TrkB/CREB/BDNF pathway in hippocampus

OBJECTIVE: . METHODS: Chemical constituents of MH were identified using liquid chromatography-mass spectrometry (LC-MS). Effects of MH on phosphorylated tyrosine kinase receptor B (p-TrkB), brain-derived neurotropic factor (BDNF) and phosphorylated cyclic AMP-dependent response element-binding protein (p-CREB) were assessed in reserpine-treated HT-22 cells and rat models. Antidepressant effects in rats were evaluated using forced swim, tail suspension and locomotor activity tests, along with hippocampal histopathology and molecular analyses. RESULTS: < 0.001) levels compared to reserpine-treated rats. DISCUSSIONS: models, suggesting therapeutic potential. However, further studies on additional pathways, including β-catenin/Wnt, MAPK/ERK, along with pharmacokinetics, are essential before recommending MH as an antidepressant agent.