Novel flow cytometry-based method for isolating and characterizing human coronary artery endothelial cells from catheter wires in coronary disease and microvascular dysfunction

Journal Article Accepted manuscript Novel flow cytometry-based method for isolating and characterizing human coronary artery endothelial cells from catheter wires in coronary disease and microvascular dysfunction Get access Philip Stapmanns , Philip Stapmanns Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, Germany https://orcid.org/0009-0004-4042-5975 Search for other works by this author on: Oxford Academic PubMed Google Scholar Youssef S Abdelwahed , Youssef S Abdelwahed Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, GermanyBerlin Institute of Health (BIH), 10117 Berlin, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar Christopher Wolfram , Christopher Wolfram Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, Germany https://orcid.org/0000-0002-7953-7833 Search for other works by this author on: Oxford Academic PubMed Google Scholar Elisabeth Strässler , Elisabeth Strässler Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, GermanyBerlin Institute of Health (BIH), 10117 Berlin, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar Arash Haghikia , Arash Haghikia Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, GermanyBerlin Institute of Health (BIH), 10117 Berlin, GermanyDepartment of Cardiology and Rhythmology, St. Josef-Hospital, Ruhr University Bochum, Bochum, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar Ulf Landmesser , Ulf Landmesser Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, GermanyBerlin Institute of Health (BIH), 10117 Berlin, GermanyFriede Springer Center for Cardiovascular Prevention @ Charité, Berlin, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar David Manuel Leistner , David Manuel Leistner Department of Medicine, Cardiology/Angiology, Goethe University Hospital, Frankfurt, GermanyDZHK (German Centre for Cardiovascular Research), partner Site Frankfurt Rhine-Main, Frankfurt, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar Nicolle Kränkel , Nicolle Kränkel Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, GermanyFriede Springer Center for Cardiovascular Prevention @ Charité, Berlin, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar Teresa Gerhardt Teresa Gerhardt Deutsches Herzzentrum der Charité (DHZC), Department of Cardiology, Charité Universitätsmedizin Berlin, Campus Benjamin-Franklin (CBF), 12203 Berlin, GermanyDZHK (German Centre for Cardiovascular Research) partner Site Berlin, 12203 Berlin, GermanyBerlin Institute of Health (BIH), 10117 Berlin, GermanyFriede Springer Center for Cardiovascular Prevention @ Charité, Berlin, GermanyCardiovascular Research Institute and the Department of Medicine, Cardiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA Corresponding author: Teresa Gerhardt, MD, Charité - Universitätsmedizin Berlin, Campus Benjamin Franklin, Friede Springer - Cardiovascular Prevention Center, Hindenburgdamm 30, 12200 Berlin, E-Mail: [email protected] https://orcid.org/0000-0002-6144-5279 Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, cvaf139, https://doi.org/10.1093/cvr/cvaf139 Published: 09 August 2025 Article history Received: 15 December 2024 Revision received: 28 February 2025 Accepted: 14 June 2025 Published: 09 August 2025