化学
放松(心理学)
再现性
二维核磁共振波谱
分辨率(逻辑)
T2弛豫
分析化学(期刊)
核磁共振波谱
定量分析(化学)
核磁共振
色谱法
磁共振成像
立体化学
计算机科学
放射科
医学
人工智能
物理
社会心理学
心理学
作者
Qiao Lin,Chun-Wang Meng,Jie Liu,Fei Liu,Qin‐Mei Zhou,Juan Liu,Cheng Peng,Liang Xiong
标识
DOI:10.1021/acs.analchem.2c05109
摘要
With the development of nuclear magnetic resonance (NMR) spectrometers and probes, two-dimensional quantitative nuclear magnetic resonance (2D qNMR) technology with a high signal resolution and great application potential has become increasingly accessible for the quantitation of complex mixtures. However, the requirement that the relaxation recovery time be equal to at least five times T1 (longitudinal relaxation time) makes it difficult for 2D qNMR to simultaneously achieve high quantitative accuracy and high data acquisition efficiency. By comprehensively using relaxation optimization and nonuniform sampling, we successfully established an optimized 2D qNMR strategy for HSQC experiments at the half-hour level and then accurately quantified the diester-type C19-diterpenoid alkaloids in Aconitum carmichaelii. The optimized strategy had the advantages of high efficiency, high accuracy, good reproducibility, and low cost and thus could serve as a reference to optimize 2D qNMR experiments for quantitative analysis of natural products, metabolites, and other complex mixtures.
科研通智能强力驱动
Strongly Powered by AbleSci AI