生物
转录因子
染色质
细胞生物学
抄写(语言学)
河马信号通路
先锋因素
体内
遗传学
黑腹果蝇
增强子
信号转导
基因
语言学
哲学
作者
Samuel A. Manning,Benjamin Kroeger,Qiji Deng,E. St. John Brooks,Yoshana Fonseka,Elizabeth Hinde,Ken Harvey
标识
DOI:10.1016/j.devcel.2024.04.006
摘要
The Hippo pathway is an important regulator of organ growth and cell fate. The major mechanism by which Hippo is known to control transcription is by dictating the nucleo-cytoplasmic shuttling rate of Yorkie, a transcription co-activator, which promotes transcription with the DNA binding protein Scalloped. The nuclear biophysical behavior of Yorkie and Scalloped, and whether this is regulated by the Hippo pathway, remains unexplored. Using multiple live-imaging modalities on Drosophila tissues, we found that Scalloped interacts with DNA on a broad range of timescales, and enrichment of Scalloped at sites of active transcription is mediated by longer DNA dwell times. Further, Yorkie increased Scalloped's DNA dwell time, whereas the repressors Nervous fingers 1 (Nerfin-1) and Tondu-domain-containing growth inhibitor (Tgi) decreased it. Therefore, the Hippo pathway influences transcription not only by controlling nuclear abundance of Yorkie but also by modifying the DNA binding kinetics of the transcription factor Scalloped.
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