生物
转录因子
抄写(语言学)
细胞生物学
表达式(计算机科学)
遗传学
计算生物学
基因
语言学
哲学
计算机科学
程序设计语言
作者
Jun He,Xiangru Huo,Gaofeng Pei,Zeran Jia,Yong-Bin Yan,Jiawei Yu,Haozhi Qu,Y. Xie,Junsong Yuan,Zheng Yang,Yanyan Hu,Minglei Shi,Kaiqiang You,Tingting Li,Tao Ma,Michael Q. Zhang,Sheng Ding,Pilong Li,Yinqing Li
出处
期刊:Cell
[Elsevier]
日期:2024-04-01
标识
DOI:10.1016/j.cell.2024.03.023
摘要
Summary
Precise control of gene expression levels is essential for normal cell functions, yet how they are defined and tightly maintained, particularly at intermediate levels, remains elusive. Here, using a series of newly developed sequencing, imaging, and functional assays, we uncover a class of transcription factors with dual roles as activators and repressors, referred to as condensate-forming level-regulating dual-action transcription factors (TFs). They reduce high expression but increase low expression to achieve stable intermediate levels. Dual-action TFs directly exert activating and repressing functions via condensate-forming domains that compartmentalize core transcriptional unit selectively. Clinically relevant mutations in these domains, which are linked to a range of developmental disorders, impair condensate selectivity and dual-action TF activity. These results collectively address a fundamental question in expression regulation and demonstrate the potential of level-regulating dual-action TFs as powerful effectors for engineering controlled expression levels.
科研通智能强力驱动
Strongly Powered by AbleSci AI