乙二胺四乙酸
牛血清白蛋白
磁性纳米粒子
超顺磁性
化学
表面改性
纳米颗粒
粒径
核化学
羧酸盐
色谱法
磁化
无机化学
纳米技术
材料科学
有机化学
螯合作用
物理化学
磁场
物理
量子力学
作者
Le Chen,Tian Gao,Xixi Wu,Mingyu He,Xiangyu Wang,Fei Teng,Yang Li
标识
DOI:10.1016/j.ijbiomac.2024.129617
摘要
Magnetic nanoparticles with increasing superparamagnetism and magnetic targeting have found widespread application in fields such as food and medicine. In this study, polycarboxylated magnetic nanoparticles (Fe3O4@SiO2@CS-COOH) were prepared by surface functionalizing iron tetraoxide (Fe3O4) nanoparticles with ethylenediaminetetraacetic acid (EDTA) as a modifier. The appropriate degree of functionalization modification was obtained by adjusting the EDTA concentration and the ratio of cross-linking agents. The prepared magnetic nanoparticles were analyzed with structural and property characterization. The results showed that the Fe3O4@SiO2@CS-COOH magnetic nanoparticles prepared with 4 % EDTA and cross-linking agents at a molar ratio of 3:4 were uniform in particle size, with an average size of roughly 7 nm, and possessed an abundant carboxylate content (310.8064 μmol/g) and a high magnetization intensity (35.05 emu/g). As a model protein, bovine serum albumin (BSA) was immobilized on the surface of magnetic particles. The largest amount of immobilized protein was 500.4376 mg BSA/g at pH 4.0 and no extra salt ions. According to molecular docking simulations, its immobilization was due to the interaction of amino and carboxyl groups at the Fe3O4@SiO2@CS-COOH/BSA interface. Fe3O4@SiO2@CS-COOH possesses a large number of carboxyl groups, strong protein immobilization, and magnetic responsiveness, which may have potential applications in biomedical and food fields.
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