费斯特共振能量转移
纳米孔
材料科学
表面等离子共振
荧光
接受者
等离子体子
生物传感器
纳米技术
分析化学(期刊)
光电子学
化学
光学
有机化学
纳米颗粒
物理
凝聚态物理
作者
L Cui,Ling Zhang,Zhexiao Li,Zhiyu Jing,Lihong Huang,Heping Zeng
出处
期刊:Nanotechnology
[IOP Publishing]
日期:2024-02-22
卷期号:35 (19): 195709-195709
标识
DOI:10.1088/1361-6528/ad20a0
摘要
Fluorescence resonance energy transfer (FRET) was found strongly enhanced by plasmon resonance. In this work, Nanoporous Gold with small amount of residual silver was used to form nanoporous gold/organic molecular layer compound with PSS and PAH. The ratio of its specific gold and silver content is achieved by controlling the time of its dealloying. Layered films of polyelectrolyte multilayers were assembled between the donor-acceptor pairs and NPG films to control distance. The maximum of FRET enhancement of 80-fold on the fluorescence intensity between the donor-acceptor pairs (CFP-YFP) is observed at a distance of ∼10.5 nm from the NPG film. This Nanoporous Gold with small amount of residual silver not only enhanced FRET 4-fold more than nanoporous gold of only gold content almost, but also effectively realized the regulation of FRET enhancement. The ability to precisely measure and regulate the enhancement of FRET enables the rational selection of plasmonic nanotransducer dimensions for the particular biosensing application.
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