Development of crosslinker-free antibody–enzyme conjugates for immunoassays in pesticide residue detection

. Recovery rates for ACE in spiked fruit samples were consistent with those obtained using liquid chromatography-mass spectrometry (LC-MS). The proposed method not only provides a reliable alternative for HRP and antibody conjugation but also advances green analytical chemistry by minimizing the use of hazardous chemicals, reducing derivative waste, and improving energy efficiency.