Improved mechanism of the bioactive compounds isolated from Lactobacillus plantarum TWK10-fermented soymilk in periodontal disease

(Aa). TWK10FSEE disrupted the cell membrane integrity, altered membrane permeability, and inhibited the growth of these periodontal pathogens. In a drip-flow biofilm reactor simulating the oral environment, TWK10FSEE regulated biofilm-related gene expression, reduced fimbriae and extracellular polysaccharide production, and retarded biofilm formation. Structural identification revealed succinic acid (SA) and a mixture of daidzein and genistein (MDG) as the primary antimicrobial components. Both SA and MDG effectively inhibited Pg and Aa growth and biofilm formation. TWK10FSEE shows potential as a functional ingredient for periodontal health.