山奈酚
黄单胞菌
抗菌活性
EC50型
微生物学
细菌
类黄酮
稻黄单胞菌
生物测定
生物
化学
最小抑制浓度
病菌
抗菌剂
生物化学
体外
抗氧化剂
遗传学
作者
An‐Ping Li,Yi He,Shaoyong Zhang,Yan‐Ping Shi
标识
DOI:10.1016/j.pestbp.2022.105221
摘要
As the most difficult to control in plant disease, phytopathogenic bacteria cause huge losses to agricultural products and economy worldwide. However, the commercially available bactericides are few and enhance pathogen resistance. To alleviate this situation, 50 flavonoids were evaluated for their antibacterial activities and mechanism of action against two intractable plant bacterial pathogens. The results of bioassays showed that most of the flavonoids exhibited moderate inhibitory effects against Xanthomonas oryzae (Xo) and Xanthomonas axonopodis pv citri (Xac). Remarkably, kaempferol showed excellent antibacterial activity against Xo in vitro (EC50 = 15.91 μg/mL) and quercetin showed the best antibacterial activity against Xac in vitro (EC50 = 14.83 μg/mL), which was better than thiodiazole copper (EC50 values against Xo and Xac were 16.79 μg/mL, 59.13 μg/mL, respectively). Subsequently, in vivo antibacterial activity assay further demonstrated kaempferol exhibited a stronger control effect on bacterial infections than thiodiazole copper. Then, the preliminary antibacterial mechanism of kaempferol was investigated by ultrastructural observations, transcriptomic, qRT-PCR analysis and biochemical index determination. These results showed that kaempferol mainly exerted bacteriostatic effects at the molecular level by affecting bacterial energy metabolism, reducing pathogenicity, and leading to disruption of cellular integrity, leakage of contents and cell death eventually.
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