TFEB
相扑蛋白
巨噬细胞
低密度脂蛋白受体
化学
细胞生物学
自噬
泡沫电池
脂蛋白
生物
体外
胆固醇
生物化学
泛素
细胞凋亡
基因
作者
Kezhou Wang,Zhou Wei,Gaolei Hu,Lifeng Wang,Rong Cai,Tian Tian
出处
期刊:Research Square - Research Square
日期:2023-06-27
标识
DOI:10.21203/rs.3.rs-3034706/v1
摘要
Abstract Atherosclerosis (AS) is a serious cardiovascular disease. One of its hallmarks is hyperlipidemia. Inhibiting the formation of macrophage foam cells is critical for alleviating AS. Transcription factor EB (TFEB) can limit the formation of macrophage foam cells by up-regulating lysosomal activity. We examined whether TFEB SUMOylation is involved in this progress during AS. In this study, we investigated the role of TFEB SUMOylation in macrophages in AS using TFEB SUMOylation deficiency Ldlr −/− (TFEB-KR: Ldlr −/− ) transgenic mice and TFEB-KR bone marrow–derived macrophages. We observed that TFEB-KR: Ldlr −/− atherosclerotic mice had thinner plaques and macrophages with higher lysosomal activity when compared to WT: Ldlr −/− mice. TFEB SUMOylation in macrophages decreased after oxidized low-density lipoprotein (OxLDL) treatment in vitro . Compared with wild type macrophages, TFEB-KR macrophages exhibited less lipid deposition after OxLDL treatment. Our study demonstrated that in AS, deSUMOylation of TFEB could inhibit the formation of macrophage foam cells through enhancing lysosomal biogenesis and autophagy, further reducing the accumulation of lipids in macrophages, and ultimately alleviating the development of AS. Thus, TFEB SUMOylation can be a switch to modulate macrophage foam cells formation and used as a potential target for AS therapy.
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