Separation of pigments from Petunia's petals using thin layer chromatography.

Anthocyanins are a group of water –soluble pigments found in fruits, vegetables and flowers that give these plants their brilliant colors. The separation of the pigments present in different petals of Petunia hybrida was conducted utilizing thin layer chromatography (TLC). The pigments were extracted from different Petunia’s petals (purple, white and violet) with ethanol prior to being spoted on the TLC plate. The characteristic color of Petunia’s petals is due to a mixture of pigments.