膜蛋白
整体膜蛋白
膜
牛分枝杆菌
生物
细菌
生物化学
外周膜蛋白
跨膜蛋白
结核分枝杆菌
酶
化学
肺结核
病理
受体
医学
遗传学
作者
Hiwa Målen,Frode S. Berven,Tina Søfteland,Magnus Ø. Arntzen,Clive S. D’Santos,Gustavo Souza,Harald G. Wiker
出处
期刊:Proteomics
[Wiley]
日期:2008-04-29
卷期号:8 (9): 1859-1870
被引量:50
标识
DOI:10.1002/pmic.200700528
摘要
Tuberculosis is an ancient disease that remains a significant global health problem. Because many membrane and membrane-associated proteins of this pathogen represent potential targets for drugs, diagnostic probes or vaccine components, we have analysed Mycobacterium bovis, bacillus Calmette-Guérin (BCG) substrain Moreau, using Triton X-114 for extraction of lipophilic proteins, followed by identification with LC coupled MS/MS. We identified 351 different proteins in total, and 103 (29%) were predicted as integral membrane proteins with at least one predicted transmembrane region and another 84 (23.9%) proteins had a positive grand average of hydropathicity (GRAVY) value, indicating increased probability for membrane association. Altogether 43 predicted lipoproteins (Lpps) were identified which is close to 50% of the total number of Lpps in the genome. Fifty-four proteins, including twenty-four predicted integral membrane proteins and seven predicted Lpps are described for the first time. The proportion of hydrophobic membrane and membrane-associated proteins shows that Triton X-114 is a highly efficient method for extraction of membrane proteins from bacteria, without the need for preisolation of membranes. ATP synthase, NAD(P) transhydrogenase, ubiquinone oxidoreductase and ubiquinol-cytochrome C reductase appear to represent major enzyme complexes in the membrane of Mycobacterium tuberculosis complex organisms.
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