过氧化氢
木质素
干旱胁迫
抗旱性
抗性(生态学)
化学
压力(语言学)
植物
生物
生物化学
农学
有机化学
语言学
哲学
作者
Yan Yu,Peng Wang,Yi Lu,Yujing Bai,Yunxie Wei,Guoyin Liu,Haitao Shi
出处
期刊:Plant Journal
[Wiley]
日期:2021-05-22
卷期号:107 (3): 847-860
被引量:66
摘要
SUMMARY Cassava, an important food and energy crop, is relatively more resistant to drought stress than other crops. However, the molecular mechanism underlying this resistance remains elusive. Herein, we report that silencing a drought stress‐responsive transcription factor MeRAV5 significantly reduced drought stress resistance, with higher levels of hydrogen peroxide (H 2 O 2 ) and less lignin during drought stress. Yeast two‐hybrid, pull down and bimolecular fluorescence complementation (BiFC) showed that MeRAV5 physically interacted with peroxidase (MePOD) and lignin‐related cinnamyl alcohol dehydrogenase 15 (MeCAD15) in vitro and in vivo . MeRAV5 promoted the activities of both MePOD and MeCAD15 to affect H 2 O 2 and endogenous lignin accumulation respectively, which are important in drought stress resistance in cassava. When either MeCAD15 or MeRAV5 was silenced, or both were co‐silenced, cassava showed lower lignin content and drought‐sensitive phenotype, whereas exogenous lignin alkali treatment increased drought stress resistance and alleviated the drought‐sensitive phenotype of these silenced cassava plants. This study documents that the modulation of H 2 O 2 and lignin by MeRAV5 is essential for drought stress resistance in cassava.
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