斑马鱼
达尼奥
小桶
转录组
生物
发育毒性
神经毒性
基因
细胞生物学
毒性
男科
基因表达
遗传学
内科学
医学
怀孕
妊娠期
作者
Yongfeng Wang,Zhiquan Yu,Zunpan Fan,Yiwei Fang,Liting He,Meili Peng,Yuanyao Chen,Zhiyong Hu,Kai Zhao,Huiping Zhang,Chunyan Liu
标识
DOI:10.1016/j.ecoenv.2021.112798
摘要
Mancozeb (MZ), an antibacterial pesticide, has been linked to reproductive toxicity, neurotoxicity, and endocrine disruption. However, whether MZ has cardiactoxicity is unclear. In this study, the cardiotoxic effects of exposure to environment-related MZ concentrations ranging from 1.88 μM to 7.52 μM were evaluated at the larval stage of zebrafish. Transcriptome sequencing predicted the mechanism of MZ-induced cardiac developmental toxicity in zebrafish by enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). Consistent with morphological changes, the osm, pfkfb3, foxh1, stc1, and nrarpb genes may effect normal development of zebrafish heart by activating NOTCH signaling pathways, resulting in pericardial edema, myocardial fibrosis, and congestion in the heart area. Moreover, differential gene expression analysis indicated that cyp-related genes (cyp1c2 and cyp3c3) were significantly upregulated after MZ treatment, which may be related to apoptosis of myocardial cells. These results were verified by real-time quantitative RT-qPCR and acridine orange staining. Our findings suggest that MZ-mediated cardiotoxic development of zebrafish larvae may be related to the activation of Notch and apoptosis-related signaling pathways.
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