PARP1
染色质
组蛋白
DNA修复
DNA损伤
ADP核糖基化
细胞生物学
DNA
生物
同源重组
核小体
聚ADP核糖聚合酶
化学
生物化学
酶
NAD+激酶
聚合酶
作者
Rebecca Smith,Siham Zentout,Catherine Chapuis,Gyula Timinszky,Sébastien Huet
标识
DOI:10.1101/2021.08.27.457930
摘要
ABSTRACT PARP1 activity is regulated by its cofactor HPF1. The binding of HPF1 on PARP1 controls the grafting of ADP-ribose moieties on serine residues of proteins nearby the DNA lesions, mainly PARP1 and histones. However, the impact of HPF1 on DNA repair regulated by PARP1 remains unclear. Here, we show that HPF1 controls both the number and the length of the ADP-ribose chains generated by PARP1 at DNA lesions. We demonstrate that HPF1-dependent histone ADP-ribosylation, rather than auto-modification of PARP1, triggers the rapid unfolding of the chromatin structure at the DNA damage sites and promotes the recruitment of the repair factors CHD4 and CHD7. Together with the observation that HPF1 contributes to efficient repair both by homologous recombination and non-homologous end joining, our findings highlight the key roles played by this PARP1 cofactor at early stages of the DNA damage response.
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