相互作用体
生物
计算生物学
转录因子
进化生物学
基因
遗传学
作者
Xi Chen,Qianya Zhu,Yumeng Nie,Fang-Xu Han,Yue Li,Harry X. Wu,Shihui Niu
出处
期刊:Forestry research
[Maximum Academic Press]
日期:2021-01-01
卷期号:1 (1): 1-12
被引量:3
标识
DOI:10.48130/fr-2021-0012
摘要
Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.
科研通智能强力驱动
Strongly Powered by AbleSci AI