Microflora of gall bladder bile in patients undergoing laparoscopic cholecystectomy

Background: Gallstone disease is one of the most common problem affecting the digestive tract. The conditions that predispose to the development of gallstones are obesity, pregnancy, dietary factors, Crohn’s disease, gastric surgery, terminal resection, hereditary spherocytosis, sickle cell disease, and thalassemia. The incidence is four times higher in women than in men with high prevalence among the age group of 30-40 years. Bacteria are commonly found in inflamed gallbladder and in patients with cholelithiasis, whereas evidence suggests that normal bile is sterile. So, exploring and identifying the microflora of gallbladder bile helps to choose appropriate antibiotics.Methods: Minimum of 50 Patients admitted under General Surgery, who are scheduled to undergo laparoscopic cholecystectomy. Five ml of bile will be aspirated from the gall bladder during laparoscopic cholecystectomy. The samples will be transferred onto a plain sterile container and then transferred at room temperature in an hour to the laboratory for culture.Results: In the present study 50 patients were evaluated for culture and gall bladder bile. Out of which maximum number of patients were clustered between 31-40 years. In the present study out of 50 patients, 34 (68%) were females and 16 (32%) were males. In the present study We found total of 33 (66%) yellow stones, 13 (26%) were black in colour and 4(8%) were brown stones. In this study bile culture was showing growth of the following organisms. The commonest organism was E. coli (64%) followed by Klebsiella (25%) and Enterococcus (11%).Conclusions: Bactibilia has long been known to be associated with biliary tract diseases and culturable bacteria in bile can represent a state of asymptomatic bactibilia which can disseminate after any intervention causing infective complication. Exploring the microflora of gall bladder bile important role in choosing the appropriate antibiotic to prevent complications.