Fractionation and Antitumor Activity of Polysaccharide from Grifola frondosa

Grifola frondosa is a edible and medical fungus which has been cultured in recent years. Polysaccharide from G. frondosa possess antitumor and immunomodulating activity. The animal test showed the crude polysaccharide mycelium and ferment liquid of G. frondosa could inhibit the growth of Sarcama 180 in mice. The inhibition ratio of GMP (polysaccharide from the mycelium) was 80.5%, whereas GLP (polysaccharide from the ferment liquid) was 58.4%～63%. The crude polysaccharides could significantly promote the function of the mouse macrophages engulfing CRBC in vivo and increase the quantity of macrophages and thymus weight in mice. The acute toxicity of crude polysaccharide and ferment liquid to mouse were tested. The results indicated that the maximum safe dose of crude polysaccharides to mouse was above 3g/kg, whereas of ferment liquid to mouse was above 30ml/kg.