Objective To analyze the application value of ultrasound, MRI and CT in the diagnosis of primary gallbladder carcinoma. Methods The imaging data of 50 patients with primary gallbladder carcinoma confirmed by operation and pathology were retrospectively analyzed. All patients underwent ultrasound examination. 40 cases underwent CT examination and 25 cases underwent MRI examination. The imaging findings were observed and analyzed, respectively. Meanwhile, the findings were compared with the results of operation and pathological examination. The accuracy of the three kinds of examinations in the diagnosis of primary gallbladder carcinoma was compared. ResultsCompared with operation and pathological results, the diagnostic accordant rates(accuracy) of ultrasound, CT and MRI were 64.0%, 73.3% and 88.0%. The diagnostic accuracy of MRI was significantly higher than that of ultrasound, and the difference was statistically significant(P0.05). Conclusion Ultrasound, MRI and CT in the diagnosis of primary gallbladder carcinoma have their own advantages and disadvantages, and the diagnostic accordant rates of MRI was the highest while of ultrasound was the lowest.