Mechanism Involved in the Apoptosis of Human Umbilical Vein Endothelial Cells Induced by Proteasome Inhibitor MG132

Objective To study the effect of proteasome inhibitor MG132 on expression of p53 in the apoptosis of cultured human umbilical vein endothelial cells induced.Methods Human umbilical vein endothelial cells were treated with MG132(2 μmol/L,5 μmol/L) for 24 hours.The apoptotic cells were determined by DNA fragment analysis and flow cytometric analysis.p53 protein expression was detected by immunohistochemistry.Results The results showed that the increase of the degree of human umbilical vein endothelial cells apoptosis was concentration dependent.MG132 could up-regulate the protein expression of p53.Conclusions The results implicated that proteasome inhibitor MG132 induced human umbilical vein endothelial cells apoptosis by inhibitting UPP and accumulation of p53.