麻风分枝杆菌
抗原
单克隆抗体
牛分枝杆菌
生物
结核分枝杆菌
微生物学
Pan-T抗原
抗体
分枝杆菌
表位
肺结核
分子生物学
病毒学
麻风病
免疫学
细菌
医学
遗传学
病理
作者
Britton Wj,L Hellqvist,Anthony Basten
出处
期刊:PubMed
日期:1986-12-01
卷期号:54 (4): 545-55
被引量:1
摘要
Monoclonal antibodies (Mabs) raised against Mycobacterium leprae sonicate defined two different determinants on related, cell-wall-associated, carbohydrate antigens common to M. leprae, M. bovis (BCG), and M. tuberculosis. Antigen inhibition ELISA and antigen capture assays demonstrated that the two antigens were present in a cell-wall fraction, M. leprae resonicate. There was species variation in the distribution of the antigens; the 4.5-6 kD antigen was more abundant in M. tuberculosis and M. bovis, while the 30-40 kD antigen was more concentrated in M. leprae preparations. Although both were present in the cell wall, only determinants on the 30-40 kD antigen were accessible on intact bacilli. The results from capture assays and Mab affinity chromatography with both L9 and L4 indicated that the 4.5-6 kD antigen was probably a fragment of the larger molecule. Both antigens are significant immunogens in the human B-cell response to M. leprae.
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