Effect of resveratrol derived from Rhizoma polygoni Cuspidati on apoptosis of gastric cancer cells HGC27

AIM: To explore the inductive effects of the resveratrol from Rhizoma polygoni Cuspidati on the cell cycles, growth and apoptosis of the gastric cancer cells HGC27. METHODS: MTT method and soft agar assay were performed to observe the effects of the resveratrol on the cell growth. Annxin-V PI staining assay and FCM assay were used to examine the inductive effects of resveratrol on the cell cycles and apoptosis. RESULTS: MTT results manifested that the cell survival rate decreased significantly and in dose dependent manner 24 h after HGC27 was incubated with 0.5 mmol/L resveratrol (P0.01 vs control). Soft agar assay results indicated that HGC27 could not form cell colony when it was incubated with 0.5 mmol/L resveratrol. Annxin-V PI staining assay and FCM assay showed that resveratrol could inhibit the HGC27 to G1 phase and induce cells to apoptosis (8.39%). CONCLUSION: Resveratrol can inhibit the growth of HGC27 and induce the cells to apoptosis.