Purification of Paeonol from Extracts Cortex Moutan

Methods of extraction and purification of paeonol from the extracts of cortex moutan were studied.CC-MS was used to measure the content of paeonol in the extract.It was found that with the combination of solvent extraction and acid and base isolation methods,the extracts with a paeonol content of 87.7% could be purified to 93%,which would be further purified by recrystalization method with a purity of 98.5%.The purified paeonol had the same Rf value with authentic sample in the thin layer chromatographic test.Paeonol obtained and authentic sample exhibited same UV absorption in 211.80 nm and 274.20 nm.