Effect of Basic Fibroblast Growth Factor on VEGF Expression in Neonate Rats Following Hypoxia-ischemia Brain Damage

Objective To explore influence and significance of exogenous bFGF on VEGF expression in hypoxia-ischemia brain of neonatal SD rat.Methods Seventy-two SD rats of 7-day-old were randomly assigned into a sham-operation group,a HIBD group and a bFGF intervention group(n=24 each).Each group was randomly subdivided into subgroups sacrificed immediately,at 3,12and 24 hrs and 3 and 7 days after HI(n=4).Expressions of VEGF were examined with immunohistochemical staining and image quantitative analysis.Results VEGF was not expressed in the hippocampus and cortex in the sham-operated group.It is obviously that the number of VEGF positive cells in the hippocampus and cortex increased reactively after HI compared with the control group(p0.05) and reached peak 12 hours and persistent to 24 hours.Exogenous bFGF enhanced the expression of VEGF in hippocampus and cortex of the neonatal rat 3 hours to 3 days after ischemia and hypoxia(p0.05).Conclusion Exogenous bFGF can up-regulate the VEGF expression in neonate rats following HIBD,which may play important roles in restoration of neurons damaged by hypoxia-ischemia.