Determination of total flavonoids and naringin in pummelo pericarp of various species from different habitats

Objective:To determine the contents of total flavonoids and naringin in pummelo pericarp from various species and producing areas of China,and to study the feasibility of extracting naringin from pummelo pericarp.Methods: The contents of total flavonoids were measured at 570 nm on a UV spectrophotometer.The contents of naringin were measured by the method of HPLC.Methanol extracts of samples and naringin reference substence were separated on a AichromBond-1 C18 column(4.6 mm×250 mm,5 μm) using a mixture solution of CH3OH-H2O-HOAC(v∶v∶v=35∶61∶4) as mobile phase and detected by UV detector at a wavelength of 283 nm.Results: The contents of total flavonoids and naringin in Exocarpium Citri Grandis are the highest,followed by the ripe fruit like Citrus paradisi cv.Changshanhuyou.,and pummelo pericarp at least.Conclusions: With this method,extracting naringin from Exocarpium Citri Grandis is optimal,and pummelo pericarp and Citrus Paradisi cv.Changshanhuyou can also be used.