Roles of BCG in establishing collagen-induced arthritis mice

Objective:To determine the correlation of BCG concentration in Freund complete adjuvant(FCA) and severity of arthritis during arthritis establishment in DBA1 /j male mice induced by bovine type Ⅱ collagen. Methods: CIA was induced by the immunization of DBA1 /j mice with bovine type Ⅱ collagen and BCG of various concentrations dissolved in FCA. To ascertain the effects administering the collagen booster,CIA-related features( including body weight,clinical scoring of arthritis),TNF-α in serum and the histopathological changes in the spleen and the joints regions were measured. Results: 4 mg /ml BCG induced more serious arthritis than 1 mg /ml in DBA1 /j mice after collagen exposure. Conclusion: There is a positive correlation of arthritis severity with BCG. Which indicates that selection of adjuvant with suitable BCG concentration could determine pathological outcome in CIA mouse model.