Taking cultural residue in Flammulina velutiper(Fr.) Sing industrial production as the basic material,using corncob as the main material,different combinations of residue and corncob were made to screen the cultivate formula of Pleurotus ostreatus.The results showed that the growth of Pleurotus ostreatus were different on different formulas.When the cultural residue of Flammulina velutiper(Fr.) Sing was 60%,the yield of Pleurotus ostreatus reached the highest,and the cost was 29.9% lower than the CK.