The Effect of Buyanghuawa Decoction and the Separate Compronents on the Level of IL-6 in Brain Tissue in Rats after Cerebral Ischemia

Objective:To investigate the effects of Buyanghuanwu Decoction(BD,补阳还五汤) and the separate compronents on the content of IL-6 in brain tissue in rats after cerebral ischemia. Methods:The string inserting method according to Longa's report was employed to make the model of focal cerebral ischemia. SD rats were divided randomly into the sham-operated group,the model group,the BD group,the astragus group and the blood-activating group. The content of IL-6 were measured by enzyme-linked immunosorbent assay (ELISA) methods. Results:There was no detectable level of IL-6 in brain tissue of the sham-operated group, Compared with that in the sham-operated group, the content of IL-6 in model group increased significantly.Compared with that in model group, The content of IL-6 in the BD group, the astragus group and the blood-activating group were all higher than that in the model group.the BD group had the strongest effects compared with the astragus group and the blood-activating group.Conclusion: BD could increase the content of IL-6, then alleviate the inflammary reaction after cerebral ischemia, which may be one of mechanisms of protective effects of Buyanghuanwu Decoction on cerebral ischemia injury. Effect of BD was better than that of separate components groups, which showed that combined use of blood-activating drug and qi reinforcing drug had coordinate effect.