人血清白蛋白
对映体
化学
微尺度热泳
猝灭(荧光)
保健品
荧光
立体化学
生物化学
量子力学
物理
作者
Jiayang Feng,Xinbing Zhao,Yunlong Yan,Haiyao Chen,Jia Liu,Xiangyang Li,Risong Na,Qing X Li
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-08-01
卷期号:385: 132686-132686
被引量:7
标识
DOI:10.1016/j.foodchem.2022.132686
摘要
Natural polyacetylenes occur in food and herbal plants, have a wide range of bioactivities, and are recognized as important nutraceuticals. Stipuol is a natural polyacetylene present in the edible plant Panax notoginseng. The present study was aimed to study interactions of rac-stipuol and its enantiomers with human serum albumin (HSA) using multi-spectroscopic, molecular modeling and microscale thermophoresis. Steady-state and time-resolved fluorescence spectra manifest that the fluorescence quenching mechanism is mainly static in type. The bindings of (S)-stipuol, (R)-stipuol, rac-stipuol lead to some microenvironmental and slight conformational changes of HSA. Competitive ligand displacement experiments and molecular modeling studies revealed that stipuol enantiomers bind to HSA at subdomain III (site IIA). The calculated values of Ka and Kd showed that (R)-stipuol had a stronger binding affinity than (S)-stipuol. The results are informative for use of stipuol as a nutraceutical to improve human health.
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