驯化
生物
基因组编辑
基因组
遗传多样性
作物
分子育种
植物抗病性
龙葵
植物育种
清脆的
生物技术
遗传学
基因
植物
农学
人口学
社会学
人口
作者
Agustín Zsögön,Tomáš Čermák,Emmanuel Rezende Naves,Marcela Morato Notini,Kai H. Edel,Stefan Weinl,Luciano Freschi,Daniel F. Voytas,Jörg Kudla,Lázaro Eustáquio Pereira Peres
摘要
Breeding of crops over millennia for yield and productivity has led to reduced genetic diversity. As a result, beneficial traits of wild species, such as disease resistance and stress tolerance, have been lost. We devised a CRISPR-Cas9 genome engineering strategy to combine agronomically desirable traits with useful traits present in wild lines. We report that editing of six loci that are important for yield and productivity in present-day tomato crop lines enabled de novo domestication of wild Solanum pimpinellifolium. Engineered S. pimpinellifolium morphology was altered, together with the size, number and nutritional value of the fruits. Compared with the wild parent, our engineered lines have a threefold increase in fruit size and a tenfold increase in fruit number. Notably, fruit lycopene accumulation is improved by 500% compared with the widely cultivated S. lycopersicum. Our results pave the way for molecular breeding programs to exploit the genetic diversity present in wild plants.
科研通智能强力驱动
Strongly Powered by AbleSci AI